Providing fast, confidential, and accurate testing services with certified professionals, mobile convenience, and dependable support for employers, families, and individuals.
(919) 371-5000
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At Square One Lab Testing Solutions LLC, we specialize in professional specimen collection services that support individuals, families, employers, attorneys, healthcare professionals, government agencies, behavioral health professionals, and third-party administrators (TPA’s) who all share one common need for accuracy and trust.
Our experienced collectors are deeply committed to delivering accurate, confidential, and fully compliant specimen collections while maintaining the highest standards of professionalism, integrity, and genuine customer care that every client deserves.
Our collectors meet every federal and state standard.
We bring professional testing directly to your location.
Square One Lab Testing Solutions offers a full spectrum of collection and verification services for employers, agencies, and individuals across North Carolina. Our comprehensive approach covers drug testing, Breath Alcohol Testing (BAT), DNA analysis, fingerprinting, I-9 verification, and phlebotomy services. We facilitate background screening services to support your workplace safety and hiring needs with the same level of professionalism and accuracy.
We understand that neither of our clients have identical needs, so we offer mobile on-site collections and appointments at our Durham location. Every service is backed by rigorous quality controls, certified collectors, and a genuine commitment to providing a seamless, confidential, and stress-free experience.







DNA testing is often needed during pivotal life moments, such as custody disputes, immigration cases, or personal family history research. We offer legal DNA tests with strict chain-of-custody, witnessed collection, and government ID checks for court-admissible results, plus non-legal tests for personal knowledge that are simpler and more affordable.
Our certified professional collectors preserve sample integrity from collection to lab analysis with meticulous care and precision. We provide parentage, siblings, grandparents, and ancestry DNA testing with empathy and accuracy.
DOT drug testing is a federally regulated process for safety-sensitive transportation employees in trucking, aviation, rail, transit, and maritime sectors. Our professional collectors strictly follow all requirements of 49 CFR Part 40, including chain-of-custody, temperature verification, and observed collections when necessary.
We perform the standard 5-panel screen for marijuana, cocaine, opiates, amphetamines, and PCP across pre-employment, random, post-accident, and other categories. All specimens are sent to a SAMHSA-certified laboratory, and the results are reviewed by a Medical Review Officer to ensure accurate final outcomes.
Non-DOT drug testing follows employer-defined policies rather than federal regulations, giving businesses the flexibility to customize their screening programs based on specific industry needs and risk tolerance. We provide non-DOT urine collection services for employers of all sizes across North Carolina, from small businesses to large staffing agencies and workforce programs.
Our collections follow best practices for specimen integrity and chain of custody, but they are not bound by the procedural requirements of 49 CFR Part 40, allowing employers to choose their panel size, testing frequency, and which substances to screen for. Common options include 5-panel, 10-panel, 12-panel, and alcohol testing, with extended panels available upon request.
We take the time to clean your fingers thoroughly, apply the appropriate amount of ink, and carefully roll each finger from nail to nail to capture the full ridge detail that agencies require for proper identification. Whether you are applying for a professional license, seeking employment with a government contractor, or fulfilling requirements for a foreign government, we treat every fingerprint card with the same careful attention to detail that we bring to all our collection services.
I-9 Employment Eligibility Verification is a critical requirement for every employer in the United States, and navigating the complex documentation and compliance rules can feel overwhelming, especially when you are trying to onboard new employees quickly and efficiently. The Form I-9 must be completed for every new hire to verify their identity and authorization to work in the United States. Failure to complete this form properly can result in significant fines and legal penalties for employers who do not maintain strict compliance with Department of Homeland Security regulations.
Our certified Breath Alcohol Technicians are extensively trained in the proper operation of EBT devices and follow all required procedures to ensure that every test result is accurate, legally defensible, and delivered with the professionalism you deserve. The two-step testing process begins with a screening test, and if the result is 0.02 g/210L or greater, a mandatory fifteen-minute waiting period is observed before a confirmation test is administered using the same EBT device.
Background checks are an essential part of the hiring process, helping employers make informed decisions that protect their organizations, their employees, and their reputation by confirming that candidates possess the qualifications and character they claim to have. We understand that hiring the wrong person can expose your business to significant risks, including workplace safety concerns, financial losses, legal liability, and damage to your brand reputation that can take years to repair.
Gender reveal testing is one of the most exciting moments for expecting parents, offering the opportunity to learn whether you are welcoming a baby boy or baby girl long before the traditional anatomy scan at twenty weeks. We understand that this news represents more than just a test result; it is a moment of connection, celebration, and anticipation that allows you to begin planning, choosing names, and bonding with your baby in a deeply personal and meaningful way.
Notary services play an essential role in validating important documents, deterring fraud, and ensuring that signatures on legal papers are authentic and properly witnessed for a wide variety of personal, legal, and business transactions. We understand that having a document notarized often arises during moments of significance such as buying a home, signing a business contract, executing the power of attorney, or finalizing estate planning documents that carry lasting implications for you and your family.
CPR training is one of the most valuable skills any person can learn, equipping you with the knowledge and confidence to respond effectively when someone experiences a cardiac emergency, choking, or other life-threatening situation where every second truly counts. We understand that the thought of performing CPR can feel intimidating, but our hands-on training is designed to build your skills gradually and give you the confidence to act decisively when an emergency occurs.
Phlebotomy services are an essential part of healthcare, providing the blood samples that physicians and healthcare providers rely on to make accurate diagnoses, monitor chronic conditions, and guide treatment decisions that affect your health and well-being. We understand that having blood drawn can be an anxiety-provoking experience for many people, whether due to a fear of needles, a previous difficult draw, or simply the uncertainty of what to expect during the procedure.
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We proudly provide professional laboratory testing, specimen collection, and mobile services throughout multiple North Carolina counties, delivering reliable support wherever clients need trusted assistance.
We provide clear, reliable guidance on drug, alcohol, DNA, and verification testing to help you make confident choices.
We have gathered the most frequently asked questions from our clients to help you understand our processes, requirements, and what to expect during your appointment.
We offer DOT urine, non-DOT urine, hair follicle, oral fluid, and rapid screening, with reliable laboratory confirmation.
Yes, we bring all professional testing directly to your workplace or home for maximum convenience and minimal disruption.
You can schedule online through our website, call our office directly, or request a mobile visit for convenience.
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